Growth Inhibition of Fusarium oxysporum f. sp. lycopercisi, the Causal Agent of Tomato Fusarium Wilt Disease by Nanoformulations Containing Talaromyces Flavus

Laleh Naraghi, Maryam Negahban, Asghar Heydari, Mohammad Razavi, Homayoun Afshari-Azad

Ekoloji, 2018, Issue 106, Pages: 103-112, Article No: e106006


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Remarkable researches in Iran have shown the importance of the Talaromyces flavus antagonist fungus to inhibit the growth of some of the important plant pathogens such as Rhizoctonia solani, Verticillium dahliae and Fusarium oxysporum. According to the results obtained from the previous researches, the commercialization of the bioformulations of this fungus is of particular importance. Since the marketing is considered to be important factor in order to commercialization, consideration of the type of bioformulation with easy application can greatly affect the attraction of relevant consumers and a successful marketing. Therefore, regarding the recent advances in the application of nanotechnology in different sciences, it seems necessary to study different nanoformulations of the mentioned biological agents with an emphasis on the ease of application and their efficacy in biological control of plant diseases. In this study, the preparation of nanoformulations including two types of nanocapsules (F1 and F3), a nano-emulsion (F2) and a powder form (F4) from T. flavus fungus was carried out. Three months after production, experimental evaluations of the effect of different nanoformulations and the formulation prepared based on former technical knowledge (rice bran and T. flavus) on sporulation, active population and efficiency in inhibiting colony growth of some important terrigenous disease agents including Verticillium dahliae, Fusarium oxysporum f. sp lycopersici and Fusarium oxysporum f. sp cucumerinum in a completely randomized design were conducted. These evaluations began three months after the production of nanoformulations and continued at intervals of three months for six months after production.


growth inhibition, fusarium oxysporum, nanoformulation


  • Alimi T, Ajewole OC, Olubode-Awosola, OO, Idowu EO (2006) Economic rationale of commercial organic fertilizer technology in vegetable production in Osun State of Nigeria. Journal of Applied Horticulture, 8(2): 159-164.
  • Anjali CH, Sudheer Khan S, MargulisGoshen K, Magdassi S, Mukherjee A, Chandrasekaran N (2010) Formulation of waterdispersible nanopermethrin for larvicidal applications, Ecotoxicology and Environmental Safety. 73(8): 1932-1936.
  • Budge SP, Whipps JM (2001) Potential for integrated control of Sclerotinia sclerotiorum in glasshouse lettuce using Coniothyrium minituns and reduced fungicide application. Phytopathology, 91(2): 221-227.
  • Caramez M, Damaso T, Costaterzi S, Farias AX, Pereira de Oliveira AC, Fraga ME, Couri S (2012) Selection of cellulolytic fungi isolated from diverse substrates. Brazilian Archives of Biology and Technology, 55(4): 513-520.
  • Ebrahimnejad P, Dinarvand R, Jafari MR, Tabasi SAS, Atyabi F (2011) Characterization, blood profile and biodistribution properties of surface modified PLGA nanoparticles of SN-38. International Journal of Pharmaceutics, 406(1-2): 122-127.
  • Engelelkes CA, Nuclo RL, Fravel DR (1997) Effect of carbon, Nitrogen, and C:N ratio on growth, sporulation, and biocontrol efficacy of Talaromyces flavus. Phytopathology, 87: 500-505.
  • Farhang Niya S, Naraghi L, Ommati F, Pirnia M (2015) Evaluation of the efficacy of the biological compound affected by Talaromyces flavus in controlling tomato Fusarium wilt disease in the field conditions. International Journal of Agricultural Science and Research, 5(2): 153-164.
  • Guan H, Chi D, Yu J, Li X (2008) a novel photodegradable insecticide: Preparation, characterization and properties evaluation of nano-Imidacloprid. Pestic biochem physiology, 92(2): 83-91.
  • Hammoudi O, Salman M, Abuamsha R, Ehlers R (2012) Effectiveness of bacterial and fungal isolates to control phoma lingam on oilseed rape, Brassica napus. American Journal of Plant sciences, 3(1): 773-779.
  • Husen E, Simanungkalit RDM, Suraswati R, Irawan I (2007) Characterization and quality assessment of Indonesian commercial biofertilizer. Indonesian Journal of Agricultural Science, 8(1): 31-38.
  • Johnson KB, Dileone JA (1999) Effect of antibiosis on antagonist dose-plant disease response relationships for the biological control of crown gall of tomato and cherry. Phytopathology, 89(1): 974-980.
  • Kaewchai S, Soytong K, Hyde, KD (2009) Mycofungicides and fungal biofertilizers. Fungal Diversity, 38: 25-50.
  • Kakvan N, Heydari A, Zamanizadeh HR, Naraghi L (2013) Development of new bioformulations using Trichoderma and Talaromyces fungal antagonists for biological control of sugar beet damping-off disease. Crop Protection, 53(1): 80-84.
  • Khan NT, Jameel N (2016) Antifungal activity of silver nanoparticles produced from fungus, Penicillium fellutanum at different pH. Journal of Microbial and Biochemical Technology, 8(5): 440-443.
  • Maji R, Dey N, Satapathy B, Mukherjee B, Mondal S (2014) Preparation and characterization of Tamoxifen citrate loaded nanoparticles for breast cancer therapy. International journal of nanomedicine, 9: 3107.
  • Margulis-Goshen, K, Magdassi S (2013) Nanotechnology: an advanced approach to the development of potent insecticides. Advanced Technologies for Managing Insect Pests, Springer, 295-314.
  • Naraghi L, Arjmandian A, Heydari A, Sharifi K, Afshari Azad H (2014) a comparison between carbendazim fungicide and Talaromyces flavus in controlling Verticillium wilt of potato under field conditions. International Journal of Agricultural Science and Research, 4(1): 89-100.
  • Pascual S, Melgarejo P, Magan N (1999) Production of the fungal biocontrol agent Epicoccum nigrum by solid substrate fermentation: effect of water activity on accumulation of compatible solutes. Mycopathologia, 146(1): 83-89.
  • Sargin S, Gezgin Y, Eltem R, Vardar F (2013) Micropropagule production from Trichoderma harzianum EGE-K38 using solid-state fermentation and a comparative study for drying methods. Turkish Journal of Biology, 37(1): 1-8.