Rapid Antimicrobial Susceptibility Testing of Yersinia Pestis Strains from Kazakhstan Plague Foci

Tatyana V. Meka-Mechenko, Zaurbek A. Sagiyev, Elmira Zh. Begimbayeva, Larissa E. Nekrassova, Galina G. Kovaleva, Uinkul A. Izbanova, Larissa Y. Lukhnova, Tatyana K. Kunitsa, Saule K. Umarova

Ekoloji, 2017, Issue 102, Pages: 5-11, Article No: e102002


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As far as new antibiotic-resistant Yersinia pestis strains appear in the climate of bioterrorism threats, plague susceptibility to antibacterials requires regular testing. Yersinia pestis should be put through rapid antimicrobial susceptibility tests. Since it grows slowly on agar media, the rapid in vivo test is especially required. The purpose of this research is to study Yersinia pestis strains from different Kazakhstan plague foci and to design an easy and fast method of antibiotic susceptibility estimation. All Y.pestis strains were susceptible to antibacterial agents traditionally recommended for plague treatment. All isolates were susceptible to ß-lactam antibacterials, including imipenem, fluoroquinolones, aminoglycosides and doxycycline. We have not detected any resistant Y.pestis strains during a 10-year investigation. We showed that preliminary rapid AST allows getting indices in 3-6 hours from the moment of Y. pestis isolation. Therefore, we recommend it for making a preliminary choice of antibacterial agents for plague treatment using HiComb MIC test for getting results that are more accurate. Strains resistant to antibacterials have not been detected during the 10-year research. The V.I. Ilyukhin’s method and the HiComb MIC test are recommended for rapid assessment of microorganism sensitivity to chemotherapy and should be out by anti-plague institutions into practical contexts.


Y.pestis, antibiotic susceptibility, plague foci, minimum inhibitory concentration, antibacterial agent


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